Robert H. Heflich Source Confirmed

Affiliation confirmed via AI analysis of OpenAlex, ORCID, and web sources.

High Impact

Researcher

National Center for Toxicological Research

faculty

51 h-index 290 pubs 8,614 cited

Research Areas

Carcinogens and Genotoxicity Assessment Molecular Biology Techniques and Applications Bioinformatics and Genomic Networks Pharmacological Effects and Toxicity Studies Cancer-related molecular mechanisms research Cancer Genomics and Diagnostics

Links

ORCID Lab Website Research Group

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OverviewAI-generated summary

Robert H. Heflich's research focuses on genotoxicity and carcinogenicity testing, particularly employing advanced molecular biology techniques. He investigates the mutagenicity and genotoxicity of various compounds, including N-nitrosamines, utilizing human cell models such as TK6 cells and HepaRG cell cultures in both 2D and 3D configurations. A significant aspect of his work involves the application of error-corrected next-generation sequencing (NGS) to enhance the precision and reliability of genotoxicity assessments. This approach aims to improve nonclinical testing strategies for genotoxicity and carcinogenicity, contributing to a more accurate evaluation of cancer risk. Heflich also explores the use of novel testing systems, such as human in vitro organotypic airway cultures, combined with technologies like the CometChip and Duplex Sequencing for DNA damage assessment and mutagenesis detection.

His scholarly contributions are reflected in a substantial publication record, with 290 total publications and over 8,600 citations, earning him recognition as a highly cited researcher. Heflich leads a research group and maintains an active lab website, indicating a commitment to ongoing research and mentorship. His collaborative network includes several colleagues from the National Center for Toxicological Research, such as Nan Mei, Roberta A. Mittelstaedt, Yuan Le, and Ji-Eun Seo, with whom he has co-authored multiple publications, underscoring the interconnected nature of his research endeavors.

Metrics

  • h-index: 51
  • Publications: 290
  • Citations: 8,614

Selected Publications

  • Detection of In Vivo Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes (2025) DOI
  • Mutation accumulation following extended exposure of human HepaRG cells to a genotoxic carcinogen (2025) DOI
  • Genotoxicity evaluation of ten nitrosamine drug substance-related impurities using 2D and 3D HepaRG cell models (2025) DOI
  • N-nitroso-ethylisopropylamine mutagenicity in rat liver using the cII transgenic mutation assay and duplex sequencing analysis of genomic DNA (2025) DOI
  • Comparative DNA damage induced by eight nitrosamines in primary human and macaque hepatocytes (2025) DOI
  • HESI GTTC ring trial: Concordance between Ames and rodent carcinogenicity outcomes for N-nitrosamines (NAs) with rat and hamster metabolic conditions (2025) DOI
  • Characterizing the Pulmonary Toxicity and Potential Mutagenicity of Formaldehyde Fumes in a Human Bronchial Epithelial Tissue Model (2025) DOI
  • Integration of the rat liver micronucleus assay into a 28-day treatment protocol: testing the genotoxicity of 4 small-molecule nitrosamines with different carcinogenic potencies and tumor target specificities (2025) DOI
  • Mutagenicity and genotoxicity evaluation of 15 nitrosamine drug substance-related impurities in human TK6 cells (2024) DOI
  • Optimizing the detection of N-nitrosamine mutagenicity in the Ames test (2024) DOI
  • Repeat treatment of organotypic airway cultures with ethyl methanesulfonate causes accumulation of somatic cell mutations without expansion of bronchial-carcinoma-specific cancer driver mutations (2024) DOI
  • <scp>Severity of effect considerations regarding the use of mutation as a toxicological endpoint for risk assessment: A report from the 8th International Workshop on Genotoxicity Testing</scp> (<scp>IWGT</scp>) (2024) DOI
  • Evaluating the mutagenicity of N-nitrosodimethylamine in 2D and 3D HepaRG cell cultures using error-corrected next generation sequencing (2024) DOI
  • Erythrocyte <i>PIG‐A</i> mutant frequencies in cancer patients receiving cisplatin (2024) DOI
  • Interpretation of in vitro concentration‐response data for risk assessment and regulatory decision‐making: Report from the 2022 <scp>IWGT</scp> quantitative analysis expert working group meeting (2023) DOI

Collaborators

Researchers in the database who share publications

Nan Mei National Center for Toxicological Research 10 shared publications Roberta A. Mittelstaedt National Center for Toxicological Research 7 shared publications Yuan Le National Center for Toxicological Research 6 shared publications Ji‐Eun Seo National Center for Toxicological Research 6 shared publications Barbara L. Parsons National Center for Toxicological Research 6 shared publications Xuefei Cao National Center for Toxicological Research 5 shared publications Mason G. Pearce National Center for Toxicological Research 5 shared publications Matthew Bryant National Center for Toxicological Research 4 shared publications

Similar Researchers

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Meagan B. Myers National Center for Toxicological Research
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Barbara L. Parsons National Center for Toxicological Research
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Azra Dad National Center for Toxicological Research
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Nadia I. Georgieva University of Arkansas for Medical Sciences
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Page B. McKinzie National Center for Toxicological Research
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Mason G. Pearce National Center for Toxicological Research
Carcinogens and Genotoxicity Assessment Molecular Biology Techniques and Applications Pharmacological Effects and Toxicity Studies
Good match 4 shared topics